Redox cycling and kinetic analysis of single molecules of solution-phase nitrite reductase

Single-molecule measurements are a valuable tool for revealing details of enzyme mechanisms by enabling observation of unsynchronized behavior. However, this approach often requires immobilizing the enzyme on a substrate, a process which may alter enzyme behavior. We apply a microfluidic trapping de...

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Bibliographic Details
Main Authors: Goldsmith, Randall H., Tabares, Leandro C., Kostrz, Dorota, Dennison, Christopher, Aartsma, Thijs J., Canters, G. W., Moerner, W. E.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2011
Subjects:
Physical Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3198337/
https://ncbi.nlm.nih.gov/pubmed/21969548
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1113572108
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