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Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus

A central circadian (~24 hr) clock coordinating daily rhythms in physiology and behavior resides in the suprachiasmatic nucleus (SCN) located in the anterior hypothalamus. The clock is directly synchronized by light via the retina and optic nerve. Circadian oscillations are generated by interacting...

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Detalhes bibliográficos
Main Authors: Savelyev, Sergey A., Larsson, Karin C., Johansson, Anne-Sofie, Lundkvist, Gabriella B. S.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2011
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3197397/
https://ncbi.nlm.nih.gov/pubmed/21372784
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2439
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