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Primary Cell Cultures from Drosophila Gastrula Embryos

Here we describe a method for preparing and culturing primary cells dissociated from Drosophila gastrula embryos. In brief, a large amount of staged embryos from young and healthy flies are collected, sterilized, and then physically dissociated into a single cell suspension using a glass homogenizer...

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Détails bibliographiques
Auteurs principaux: Perrimon, Norbert, Zirin, Jonathan, Bai, Jianwu
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2011
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3197384/
https://ncbi.nlm.nih.gov/pubmed/21403631
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2215
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