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Detection of Signaling Effector-Complexes Downstream of BMP4 Using in situ PLA, a Proximity Ligation Assay

BMPs are responsible for a wide range of developmental and biological effects. BMP receptors activate (phosphorylate) the Smad1/5/8 effectors, which then, form a complex with Smad4 and translocate to the nucleus where they function as transcription factors to initiate BMP specific downstream effects...

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Detalhes bibliográficos
Main Authors: Thymiakou, Efstathia, Episkopou, Vasso
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3197320/
https://ncbi.nlm.nih.gov/pubmed/21403637
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2631
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