IgY Technology: Extraction of Chicken Antibodies from Egg Yolk by Polyethylene Glycol (PEG) Precipitation

Hens can be immunized by means of i.m. vaccination (Musculus pectoralis, left and right, injection volume 0.5-1.0 ml) or by means of Gene-Gun plasmid-immunization. Dependent on the immunogenicity of the antigen, high antibody-titres (up to 1:100,000 - 1:1,000,000) can be achieved after only one or 3...

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Bibliografske podrobnosti
Main Authors: Pauly, Diana, Chacana, Pablo A., Calzado, Esteban G., Brembs, Björn, Schade, Rüdiger
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2011
Teme:
Immunology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3197133/
https://ncbi.nlm.nih.gov/pubmed/21559009
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/3084
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