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Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

Two electrode voltage clamp electrophysiology (TEVC) is a powerful tool to investigate the mechanism of ion transport1 for a wide variety of membrane proteins including ion channels(2), ion pumps(3), and transporters(4). Recent developments have combined site-specific fluorophore labeling alongside...

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Detalhes bibliográficos
Main Authors: Richards, Ryan, Dempski, Robert E.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3197104/
https://ncbi.nlm.nih.gov/pubmed/21673634
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2627
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