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Photobleaching Assays (FRAP & FLIP) to Measure Chromatin Protein Dynamics in Living Embryonic Stem Cells

Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Loss In Photobleaching (FLIP) enable the study of protein dynamics in living cells with good spatial and temporal resolution. Here we describe how to perform FRAP and FLIP assays of chromatin proteins, including H1 and HP1, in mouse...

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Autori principali: Nissim-Rafinia, Malka, Meshorer, Eran
Natura: Artigo
Lingua:Inglês
Pubblicazione: MyJove Corporation 2011
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3197042/
https://ncbi.nlm.nih.gov/pubmed/21730953
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2696
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