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Photobleaching Assays (FRAP & FLIP) to Measure Chromatin Protein Dynamics in Living Embryonic Stem Cells

Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Loss In Photobleaching (FLIP) enable the study of protein dynamics in living cells with good spatial and temporal resolution. Here we describe how to perform FRAP and FLIP assays of chromatin proteins, including H1 and HP1, in mouse...

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書目詳細資料
Main Authors: Nissim-Rafinia, Malka, Meshorer, Eran
格式: Artigo
語言:Inglês
出版: MyJove Corporation 2011
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3197042/
https://ncbi.nlm.nih.gov/pubmed/21730953
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2696
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