Fluorescence image screening for chemical compounds modifying cholesterol metabolism and distribution

An automated fluorescence microscopy assay using a nontoxic cholesterol binding protein, θ toxin domain 4, (D4), was developed in order to identify chemical compounds modifying intracellular cholesterol metabolism and distribution. Using this method, we screened a library of 1,056 compounds and iden...

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Bibliografiska uppgifter
Huvudupphovsmän: Ishitsuka, Reiko, Saito, Tamio, Osada, Hiroyuki, Ohno-Iwashita, Yoshiko, Kobayashi, Toshihide
Materialtyp: Artigo
Språk:Inglês
Publicerad: The American Society for Biochemistry and Molecular Biology 2011
Ämnen:
Methods
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3196239/
https://ncbi.nlm.nih.gov/pubmed/21862703
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D018184
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