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Probing the molecular structure of phytochrome with immobilized Cibacron blue 3GA and blue dextran

Phytochrome was shown to bind to agarose-immobilized Cibacron blue 3GA. A higher affinity of the dye for the putative biologically active form (P(fr)) than the inactive form (P(r)) of phytochrome was observed. Effective general eluants of P(r) included 40% (vol/vol) ethylene glycol, 1% Triton X-100,...

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Publicat a:Proc Natl Acad Sci U S A
Autor principal: Smith, William O.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 1981
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC319482/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593016/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.5.2977
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