Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli.
Sau 3A1 partial digestion fragments from Bacillus thuringiensis var. kurstaki HD-1 plasmid DNA were ligated into the BamHI site of the cloning vector pBR322 and transformed into Escherichia coli strain HB101. Colonies presumed to contain recombinant plasmids were screened for production of an antige...
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| Udgivet i: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
National Academy of Sciences
1981
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC319465/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7019914/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.5.2893 |
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