Determination of cellular RNA concentrations by electron microscopy of R loop-containing DNA.
R loop hybridizations and electron microscopy have been used to determine cellular RNA concentrations for cloned genes. In plasmid DNA sequence excess, all the complementary RNA is driven into R loop structures that can be assayed by electron microscopy. To determine the concentration of a particula...
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| Опубликовано в:: | Proc Natl Acad Sci U S A |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
National Academy of Sciences
1981
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC319449/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6265914/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.5.2820 |
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