Kinetic Asymmetry of Subunit Exchange of Homooligomeric Protein as Revealed by Deuteration-Assisted Small-Angle Neutron Scattering

We developed a novel, to our knowledge, technique for real-time monitoring of subunit exchange in homooligomeric proteins, using deuteration-assisted small-angle neutron scattering (SANS), and applied it to the tetradecamer of the proteasome α7 subunit. Isotopically normal and deuterated tetradecame...

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Autors principals: Sugiyama, Masaaki, Kurimoto, Eiji, Yagi, Hirokazu, Mori, Kazuhiro, Fukunaga, Toshiharu, Hirai, Mitsuhiro, Zaccai, Giuseppe, Kato, Koichi
Format: Artigo
Idioma:Inglês
Publicat: The Biophysical Society 2011
Matèries:
Protein
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3192978/
https://ncbi.nlm.nih.gov/pubmed/22004758
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.09.004
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