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Kinetics of Luminal Proton Binding to the SR Ca-ATPase

An open membrane preparation containing SR Ca-ATPase was prepared from sarcoplasmic-reticulum vesicles to study the ion binding kinetics in the P-E(2) conformation. Because Ca(2+) and H(+) binding are electrogenic reactions, fluorescent styryl dyes could be used to determine changes in the binding s...

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Detalles Bibliográficos
Main Authors: Fibich, Andreas, Apell, Hans-Jürgen
Formato: Artigo
Idioma:Inglês
Publicado: The Biophysical Society 2011
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3192968/
https://ncbi.nlm.nih.gov/pubmed/22004743
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2011.09.014
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