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Reconstituted Escherichia coli Bam complex catalyzes multiple rounds of β-barrel assembly
β-barrel proteins are folded and inserted into the outer membranes of E. coli by the multi-protein Bam complex. The Bam complex has been purified and functionally reconstituted in vitro. Here we report assay conditions for this reconstitution that increase the folding yield ten-fold and allow us to...
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| Asıl Yazarlar: | , |
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| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
2011
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3192448/ https://ncbi.nlm.nih.gov/pubmed/21823654 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi2010784 |
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