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Reconstituted Escherichia coli Bam complex catalyzes multiple rounds of β-barrel assembly

β-barrel proteins are folded and inserted into the outer membranes of E. coli by the multi-protein Bam complex. The Bam complex has been purified and functionally reconstituted in vitro. Here we report assay conditions for this reconstitution that increase the folding yield ten-fold and allow us to...

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Bibliografiska uppgifter
Huvudupphovsmän: Hagan, Christine L., Kahne, Daniel
Materialtyp: Artigo
Språk:Inglês
Publicerad: 2011
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3192448/
https://ncbi.nlm.nih.gov/pubmed/21823654
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi2010784
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