Enzyme-linked sandwich immunoassay for insulin using laser fluorimetric detection.
Human serum samples are assayed for insulin by an enzyme-linked sandwich immunoassay. Horseradish peroxidase is used as an enzyme label for antibody, and enzyme activity is measured by means of the fluorogenic substrate, p-hydroxyphenylacetic acid. The product is detected by excitation of fluorescen...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1981
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC319243/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7015349/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.78.3.1901 |
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