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Role of α-Helical Structure in Organic Solvent-Activated Homodimer of Elastase Strain K
Recombinant elastase strain K overexpressed from E. coli KRX/pCon2(3) was purified to homogeneity by a combination of hydrophobic interaction chromatography and ion exchange chromatography, with a final yield of 48% and a 25-fold increase in specific activity. The purified protein had exhibited a fi...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Molecular Diversity Preservation International (MDPI)
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3189751/ https://ncbi.nlm.nih.gov/pubmed/22016627 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/ijms12095797 |
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