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Transcription of a cloned rainbow trout protamine gene is accurately initiated following transfection into HeLa cells but the majority of the transcripts fail to polyadenylate at the correct site.
The expression of a cloned trout protamine gene transfected into mammalian cells in culture has been studied. This small intronless gene has a consensus TATA-box, a classical AATAAA sequence and the cap and polyadenylation sites are separated by only 228 base pairs (Gregory et al., ref 10). When 1kb...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1985
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC318946/ https://ncbi.nlm.nih.gov/pubmed/4080552 |
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