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Heterogeneity of mammalian DNA ligase detected on activity and DNA sequencing gels.
A new method to detect DNA ligase activity in situ after NaDodSO4 polyacrylamide gel electrophoresis has been developed. After renaturation of active polypeptides the ligase reaction occurs in situ by incubating the intact gel in the presence of Mg++ and ATP. Further treatment with alkaline phosphat...
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| Main Authors: | , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
1984
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| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC318904/ https://ncbi.nlm.nih.gov/pubmed/6377238 |
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