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Expression of antigenic regions of a trypanosome variable surface glycoprotein in E. coli using Bal-31 nuclease digestion.

We have used the expression of a trypanosome variable surface glycoprotein (VSG) in E. coli to produce VSG serotype-specific antisera which have none of the cross-reacting specificities characteristic of antisera prepared against purified VSGs. This was accomplished by treating restriction fragments...

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Détails bibliographiques
Auteurs principaux: Lenardo, M J, Brentano, S T, Donelson, J E
Format: Artigo
Langue:Inglês
Publié: 1984
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC318864/
https://ncbi.nlm.nih.gov/pubmed/6204274
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