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Assembly of functional antibodies from immunoglobulin heavy and light chains synthesised in E. coli.
Genes for a murine mu heavy chain and a lambda light chain immunoglobulin have been inserted into bacterial expression plasmids containing the Escherichia coli trp promoter and ribosome binding site. Induction of transcription from the trp promoter results in accumulation of both light and heavy cha...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1984
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC318790/ https://ncbi.nlm.nih.gov/pubmed/6328437 |
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