Evaluation of Two Magnetic-Bead-Based Viral Nucleic Acid Purification Kits and Three Real-Time Reverse Transcription-PCR Reagent Systems in Two TaqMan Assays for Equine Arteritis Virus Detection in Semen

This study showed that under specifically defined conditions with respect to nucleic acid extraction method and testing reagents, a previously described real-time reverse transcription-PCR (rRT-PCR) assay (T1 assay) provides sensitivity equal to or higher than that of virus isolation for the detecti...

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Bibliografische gegevens
Hoofdauteurs: Miszczak, Fabien, Shuck, Kathleen M., Lu, Zhengchun, Go, Yun Young, Zhang, Jianqiang, Sells, Stephen, Vabret, Astrid, Pronost, Stéphane, Fortier, Guillaume, Timoney, Peter J., Balasuriya, Udeni B. R.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: American Society for Microbiology 2011
Onderwerpen:
Clinical Veterinary Microbiology
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3187312/
https://ncbi.nlm.nih.gov/pubmed/21832018
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01187-11
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