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Validation of a Low-Cost Human Papillomavirus Genotyping Assay Based on PGMY PCR and Reverse Blotting Hybridization with Reusable Membranes

The genotyping of human papillomaviruses (HPV) is essential for the surveillance of HPV vaccines. We describe and validate a low-cost PGMY-based PCR assay (PGMY-CHUV) for the genotyping of 31 HPV by reverse blotting hybridization (RBH). Genotype-specific detection limits were 50 to 500 genome equiva...

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Autori principali: Estrade, C., Menoud, P.-A., Nardelli-Haefliger, D., Sahli, R.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2011
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3187290/
https://ncbi.nlm.nih.gov/pubmed/21832011
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.05039-11
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