PCR Amplification-Independent Methods for Detection of Microbial Communities by the High-Density Microarray PhyloChip

Environmental microbial community analysis typically involves amplification by PCR, despite well-documented biases. We have developed two methods of PCR-independent microbial community analysis using the high-density microarray PhyloChip: direct hybridization of 16S rRNA (dirRNA) or rRNA converted t...

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Hauptverfasser: DeAngelis, Kristen M., Wu, Cindy H., Beller, Harry R., Brodie, Eoin L., Chakraborty, Romy, DeSantis, Todd Z., Fortney, Julian L., Hazen, Terry C., Osman, Shariff R., Singer, Mary E., Tom, Lauren M., Andersen, Gary L.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2011
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Methods
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3187179/
https://ncbi.nlm.nih.gov/pubmed/21764955
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.05262-11
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