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Expression of an Mg(2+)-Dependent HIV-1 RNase H Construct for Drug Screening
A single polypeptide of the HIV-1 reverse transcriptase that reconstituted Mg(2+)-dependent RNase H activity has been made. Using molecular modeling, the construct was designed to encode the p51 subunit joined by a linker to the thumb (T), connection (C), and RNase H (R) domains of p66. This p51-G-T...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3186996/ https://ncbi.nlm.nih.gov/pubmed/21768506 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.00658-11 |
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