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DNA supercoiling determines the activation energy barrier for site specific recombination by Tn21 resolvase.
A kinetic analysis of site specific recombination by Tn21 resolvase has been carried out using DNA substrates of varying superhelicities. The rates for the formation of the recombinant product increased with increasing superhelicity up to a maximum value, after which further increases in superhelici...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1989
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC318432/ https://ncbi.nlm.nih.gov/pubmed/2550904 |
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