An efficient strategy for cloning 5' extremities of rare transcripts permits isolation of multiple 5'-untranslated regions of rat tryptophan hydroxylase mRNA.

The 5' end mapping of rat tryptophan hydroxylase (TPH) mRNA indicated a diversity in 5'-untranslated regions. Corresponding sequences were isolated by a variant of the Polymerase Chain Reaction, recently designated as 'anchor PCR', and a 'cRNA enrichment' procedure. The...

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Bibliografiska uppgifter
Huvudupphovsmän: Delort, J, Dumas, J B, Darmon, M C, Mallet, J
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1989
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC318339/
https://ncbi.nlm.nih.gov/pubmed/2780283
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