A Novel Approach to Dual Excitation Ratiometric Optical Mapping of Cardiac Action Potentials with Di-4-ANEPPS using Pulsed LED Excitation

We developed a new method for ratiometric optical mapping of transmembrane potential (V(m)) in cardiac preparations stained with di-4-ANEPPS. V(m)-dependent shifts of excitation and emission spectra establish two excitation bands (<481 and >481 nm) that produce fluorescence changes of opposite...

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Autori principali: Bachtel, Andrew D., Gray, Richard A., Stohlman, Jayna M., Bourgeois, Elliot B., Pollard, Andrew E., Rogers, Jack M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2011
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3182506/
https://ncbi.nlm.nih.gov/pubmed/21536528
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1109/TBME.2011.2148719
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