Purified U5 small nuclear ribonucleoprotein can relieve the inhibition of spliceosome assembly and splicing by snRNP-free nuclear proteins.

As demonstrated by RNase T1 protection assays at 0 degrees C without ATP, U1 and U5 snRNPs purified by isopycnic centrifugation in cesium chloride bind to the 5' and 3' splice sites of human beta-globin pre-mRNA, respectively. We also devised a saturation-complementation assay and have found that th...

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Veröffentlicht in:	Nucleic Acids Res
Hauptverfasser:	Tazi, J, Temsamani, J, Alibert, C, Rhead, W, Khellil, S, Cathala, G, Brunel, C, Jeanteur, P
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Oxford University Press 1989
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC318107/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2527352/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.13.5223
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