Efficient oligonucleotide-directed construction of mutations in expression vectors by the gapped duplex DNA method using alternating selectable markers.

An efficient method for the construction of multiple mutations in a sequential manner is described. It is based on the gapped duplex DNA approach to oligonucleotide-directed mutagenesis (Kramer et al. 1984, Nucl. Acids Res. 12, 9441-9456) and a set of newly constructed phasmid vectors. These are cha...

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Bibliographic Details
Main Authors: Stanssens, P, Opsomer, C, McKeown, Y M, Kramer, W, Zabeau, M, Fritz, H J
Format: Artigo
Language:Inglês
Published: 1989
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC318004/
https://ncbi.nlm.nih.gov/pubmed/2501754
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