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Cloning of binding sequences for the Escherichia coli transcription activators, FNR and CRP: location of bases involved in discrimination between FNR and CRP.

Expression from the E.coli melR promoter (pmelR) is normally totally dependent on the transcription activator protein, CRP. We describe experiments with a genetically engineered DNA fragment carrying pmelR in which the wild type CRP binding site was replaced with synthetic oligonucleotides containin...

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Detalhes bibliográficos
Main Authors: Bell, A I, Gaston, K L, Cole, J A, Busby, S J
Formato: Artigo
Idioma:Inglês
Publicado em: 1989
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC317865/
https://ncbi.nlm.nih.gov/pubmed/2543955
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