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Complementation of a Dictyostelium discoideum thymidylate synthase mutation with the mouse gene provides a new selectable marker for transformation.

A cDNA encoding mouse thymidylate synthase has been inserted 3' to the Dictyostelium discoideum actin 15 promoter in an E. coli-D.discoideum shuttle vector. When this construct was introduced into a D.discoideum thymidylate synthase mutant strain HPS400, stable transformants were obtained at hi...

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Main Authors: Chang, A C, Williams, K L, Williams, J G, Ceccarelli, A
Formáid: Artigo
Teanga:Inglês
Foilsithe: 1989
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC317847/
https://ncbi.nlm.nih.gov/pubmed/2734099
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