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Tracking bacterial DNA replication forks in vivo by pulsed field gel electrophoresis.

The location of chromosomal DNA replication forks was identified in synchronously replicating E. coli cultures by pulse labeling DNA at specific times with 14C-thymidine and following incorporation of radionucleotide into genomic Not I restriction fragments. This technique could be used to character...

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Détails bibliographiques
Publié dans:Nucleic Acids Res
Auteurs principaux: Ohki, M, Smith, C L
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 1989
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC317790/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2657661/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.9.3479
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