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Tracking bacterial DNA replication forks in vivo by pulsed field gel electrophoresis.
The location of chromosomal DNA replication forks was identified in synchronously replicating E. coli cultures by pulse labeling DNA at specific times with 14C-thymidine and following incorporation of radionucleotide into genomic Not I restriction fragments. This technique could be used to character...
Gardado en:
| Publicado en: | Nucleic Acids Res |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Oxford University Press
1989
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| Acceso en liña: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC317790/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2657661/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.9.3479 |
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