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Tracking bacterial DNA replication forks in vivo by pulsed field gel electrophoresis.

The location of chromosomal DNA replication forks was identified in synchronously replicating E. coli cultures by pulse labeling DNA at specific times with 14C-thymidine and following incorporation of radionucleotide into genomic Not I restriction fragments. This technique could be used to character...

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Bibliografske podrobnosti
izdano v:Nucleic Acids Res
Main Authors: Ohki, M, Smith, C L
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 1989
Online dostop:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC317790/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2657661/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.9.3479
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