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Direct genomic fluorescent on-line sequencing and analysis using in vitro amplification of DNA.

In vitro amplification of genomic DNA and total RNA, as well as recombinant DNA, using one fluorescently labelled and one unlabelled primer during amplification, together with on-line analysis of the products on the EMBL fluorescent DNA sequencer, is described. Further is reported direct sequencing...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Main Authors: Voss, H, Schwager, C, Wirkner, U, Sproat, B, Zimmermann, J, Rosenthal, A, Erfle, H, Stegemann, J, Ansorge, W
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 1989
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC317640/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2470026/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/17.7.2517
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