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Immuno-Hybridization Chain Reaction for Enhancing Detection of Individual Cytokine-Secreting Human Peripheral Mononuclear Cells

We present here a new method to enhance the detection of secreted cytokines and chemokines from single human mononuclear cells. The technique uses a hybridization chain reaction (HCR) to amplify signals resulting from sandwich immunoassays. This immuno-HCR employs oligonucleotide-based initiators co...

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Detalhes bibliográficos
Main Authors: Choi, Jonghoon, Love, Kerry Routenberg, Gong, Yuan, Gierahn, Todd M., Love, J. Christopher
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3175753/
https://ncbi.nlm.nih.gov/pubmed/21812465
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac2013916
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