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The effects of stem length and core placement on shRNA activity

BACKGROUND: Expressed short hairpin RNAs (shRNA) used in mammalian RNA interference (RNAi) are often designed around a specific short interfering RNA (siRNA) core. Whilst there are algorithms to aid siRNA design, hairpin-specific characteristics such as stem-length and siRNA core placement within th...

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Detalhes bibliográficos
Main Authors: Mcintyre, Glen J, Yu, Yi-Hsin, Lomas, Mehnaaz, Fanning, Gregory C
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3175162/
https://ncbi.nlm.nih.gov/pubmed/21819628
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-12-34
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