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Improved specificity for detection of Mycobacterium bovis in fresh tissues using IS6110 real-time PCR

BACKGROUND: Culture of M. bovis from diagnostic specimens is the gold standard for bovine tuberculosis diagnostics in the USA. Detection of M. bovis by PCR in tissue homogenates may provide a simple rapid method to complement bacterial culture. A significant impediment to PCR based assays on tissue...

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Detalhes bibliográficos
Main Authors: Thacker, Tyler C, Harris, Beth, Palmer, Mitchell V, Waters, Wade R
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3170578/
https://ncbi.nlm.nih.gov/pubmed/21867516
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1746-6148-7-50
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