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Transfection Optimization for Primary Human CD8+ Cells

Electroporation, a non-virus-mediated gene transfection method, has traditionally had poor outcomes with low gene transfection efficiency and poor cellular viability, particularly in primary human lymphocytes. Herein we have optimized the electroporation conditions for primary CD8+ cells resulting i...

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Detalhes bibliográficos
Main Authors: Liu, Lianxing, Johnson, Carl, Fujimura, Sue, Teque, Fernando, Levy, Jay A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3170435/
https://ncbi.nlm.nih.gov/pubmed/21777592
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2011.06.026
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