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Microbial DNA extraction from intestinal biopsies is improved by avoiding mechanical cell disruption
Currently, standard protocols for microbial DNA extraction from intestinal tissues do not exist. We assessed the efficiency of a commercial kit with and without mechanical disruption. Better quality DNA was obtained without mechanical disruption. Thus, it appears that bead-beating is not required fo...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2011
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3168962/ https://ncbi.nlm.nih.gov/pubmed/21820015 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2011.07.014 |
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