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Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

Gene deletion and protein overexpression are common methods for studying functions of proteins. In this article, we describe a protocol for analysis of phenotype development as a function of protein concentration at population and single-cell levels in Saccharomyces cerevisiae. Although this protoco...

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Bibliografiske detaljer
Main Authors: Mukherjee, Debarati, Sen, Arpita, Aguilar, R. Claudio
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3168202/
https://ncbi.nlm.nih.gov/pubmed/20336036
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1863
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