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Sensitive detection of nucleic acids by PNA hybridization directed co-localization of fluorescent beads

We have designed a pair of biotinylated peptide nucleic acid (PNA) probes targeting two sequences in 18S rRNA (from the parasite Trypanosoma brucei) at a distance of 191 nt (corresponding to maximum distance of ca. 60 nm) from each other. The PNA probes were individually bound to (strept)avidin-coat...

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Detalles Bibliográficos
Autores principales: Shiraishi, Takehiko, Deborggraeve, Stijn, Büscher, Philippe, Nielsen, Peter E
Formato: Artigo
Lenguaje:Inglês
Publicado: Landes Bioscience 2011
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3166491/
https://ncbi.nlm.nih.gov/pubmed/21912728
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/adna.2.2.16562
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