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Sensitive detection of nucleic acids by PNA hybridization directed co-localization of fluorescent beads
We have designed a pair of biotinylated peptide nucleic acid (PNA) probes targeting two sequences in 18S rRNA (from the parasite Trypanosoma brucei) at a distance of 191 nt (corresponding to maximum distance of ca. 60 nm) from each other. The PNA probes were individually bound to (strept)avidin-coat...
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| Autores principales: | , , , |
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| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
Landes Bioscience
2011
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3166491/ https://ncbi.nlm.nih.gov/pubmed/21912728 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/adna.2.2.16562 |
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