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Initial recognition of U12-dependent introns requires both U11/5′ splice-site and U12/branchpoint interactions

We have investigated the formation of prespliceosomal complex A in HeLa nuclear extracts on a splicing substrate containing an AT-AC (U12-type) intron from the P120 gene. Using an RNase H protection assay and specific blocking oligonucleotides, we find that recognition of the 5′ splice-site (5′ss) a...

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Detalhes bibliográficos
Main Authors: Frilander, Mikko J., Steitz, Joan A.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 1999
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC316595/
https://ncbi.nlm.nih.gov/pubmed/10197985
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