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Determination of the Catalytic Base in Family 48 Glycosyl Hydrolases
The catalytic base in family 48 glycosyl hydrolases has not been previously established experimentally. Based on structural and modeling data published to date, we used site-directed mutagenesis and azide rescue activity assays to show definitively that the catalytic base in Thermobifida fusca Cel48...
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| Asıl Yazarlar: | , |
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| Materyal Türü: | Artigo |
| Dil: | Inglês |
| Baskı/Yayın Bilgisi: |
American Society for Microbiology
2011
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| Konular: | |
| Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3165399/ https://ncbi.nlm.nih.gov/pubmed/21764975 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.05532-11 |
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