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Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

We developed a protocol to measure the bending rigidity of filamentous rod-shaped bacteria. Forces are applied with an optical trap, a microscopic three-dimensional spring made of light that is formed when a high-intensity laser beam is focused to a very small spot by a microscope's objective l...

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Detalhes bibliográficos
Main Authors: Wang, Siyuan, Arellano-Santoyo, Hugo, Combs, Peter A., Shaevitz, Joshua W.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3164081/
https://ncbi.nlm.nih.gov/pubmed/20421864
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2012
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