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Efficient implementation of RNA interference in the pathogenic yeast Cryptococcus neoformans

An improved method has been developed for RNA interference in Cryptococcus neoformans, using opposing promoters to facilitate cloning and RNA interference targeting URA5 to allow selection of cells in which silencing is most effective. These advances significantly reduce the variability of silencing...

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Main Authors: Bose, Indrani, Doering, Tamara L.
格式: Artigo
語言:Inglês
出版: 2011
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3163904/
https://ncbi.nlm.nih.gov/pubmed/21554906
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mimet.2011.04.014
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