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Vectors bicistronically linking a gene of interest to the SV40 large T antigen in combination with the SV40 origin of replication enhance transient protein expression and luciferase reporter activity
The Simian Virus large T antigen (SVLT) induces replication of plasmids bearing the SV40 origin of replication (SV40 ori) within mammalian cells. The internal ribosomal entry site (IRES) is an element that allows for the co-translation of proteins from one polycistronic mRNA. Through the combination...
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3163069/ https://ncbi.nlm.nih.gov/pubmed/21806556 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000113720 |
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