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A Multiplex RNA-seq Strategy to Profile Poly(A(+)) RNA: Application to Analysis of Transcription Response and 3′ End Formation
RNA-seq technologies are now replacing microarrays for profiling gene expression. Here we describe a robust RNA-seq strategy for multiplex analysis of RNA samples based on deep sequencing. First, an oligo-dT linked to an adaptor sequence is used to prime cDNA synthesis. Upon solid phase selection, s...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3160523/ https://ncbi.nlm.nih.gov/pubmed/21515359 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ygeno.2011.04.003 |
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