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Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Isolation and expansion of the putative neural stem cells (NSCs) from the adult murine brain was first described by Reynolds and Weiss in 1992 employing a chemically defined serum-free culture system known as the neurosphere assay (NSA). In this assay, the majority of differentiated cell types die w...

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Main Authors: Azari, Hassan, Rahman, Maryam, Sharififar, Sharareh, Reynolds, Brent A.
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3159587/
https://ncbi.nlm.nih.gov/pubmed/21113123
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/2393
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