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Generation of Induced Pluripotent Stem Cells by Reprogramming Mouse Embryonic Fibroblasts with a Four Transcription Factor, Doxycycline Inducible Lentiviral Transduction System

Using a defined set of transcription factors and cell culture conditions, Yamanaka and colleagues demonstrated that retrovirus-mediated delivery and expression of Oct4, Sox2, c-Myc, and Klf4 is capable of inducing pluripotency in mouse fibroblasts.(1) Subsequent reports have demonstrated the utility...

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Autors principals: Hamilton, Brad, Feng, Qiang, Ye, Mike, Welstead, G Grant
Format: Artigo
Idioma:Inglês
Publicat: MyJove Corporation 2009
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3157852/
https://ncbi.nlm.nih.gov/pubmed/19915522
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1447
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