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Development of a fluorogenic sensor for activated Cdc42

Cdc42, a member of the Rho GTPase family, is a fundamental regulator of the actin cytoskeleton during cell migration. To generate a sensor for Cdc42 activation, we employed a multi-pronged approach, utilizing cysteine labeling and expressed protein ligation, to incorporate the environment sensitive...

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Detalhes bibliográficos
Main Authors: Goguen, Brenda N., Loving, Galen S., Imperiali, Barbara
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3156282/
https://ncbi.nlm.nih.gov/pubmed/21549598
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bmcl.2011.04.051
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