Intranuclear Microinjection of DNA into Dissociated Adult Mammalian Neurons

Primary neuronal cell cultures are valuable tools to study protein function since they represent a more biologically relevant system compared to immortalized cell lines. However, the post-mitotic nature of primary neurons prevents effective heterologous protein expression using common procedures suc...

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Bibliographic Details
Main Authors: Lu, Van B., Williams, Damian J., Won, Yu-Jin, Ikeda, Stephen R.
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2009
Subjects:
Cellular Biology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3152246/
https://ncbi.nlm.nih.gov/pubmed/20010540
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/1614
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